Antibiotic susceptibility pattern of bacterial isolates from quantitative culture of bronchoalveolar lavage fluid in patients with clinical suspicion of pneumonia


Bronchoalveolar lavage

How to Cite

Jha, B., Sharma, M. ., Sapkota, J., Pant, S. ., & Neopane, A. (2021). Antibiotic susceptibility pattern of bacterial isolates from quantitative culture of bronchoalveolar lavage fluid in patients with clinical suspicion of pneumonia. Journal of Kathmandu Medical College, 9(4), 197–200. Retrieved from


Background: The common bacterial pathogens isolated from bronchoalveolar lavage (BAL) include Klebsiella pneumoniae, Acinetobacter baumanii, Pseudomonas aeruginosa and Coagulase negative Staphylococcus.

Objectives: This study was conducted to identify pneumonia causing organisms and to determine their antibiogram.

Methodology: A descriptive cross-sectional study was carried out at Kathmandu Medical College Teaching Hospital, Kathmandu, Nepal over a period of one year (Aug 2019-Jul 2020). Ethical approval (Reference Number:1004201810) was taken and convenience sampling was done using Clinical and Laboratory Standards Institute (CLSI) guidelines. Data were analyzed using Statistical Package for Social Sciences(SPSS) version 19.

Results: Out of 32 BAL samples, 14 were culture positive. The commonest bacterial pathogens isolated were Klebsiella Pneumoniae 6(42.85%), followed by Acinetobacter baumanii 5(35.71%), Pseudomonas aeruginosa 2(14.28%) and Coagulase negative Staphylococcus 1(7.14%). The isolates were 100% sensitive to Tigecycline and Polymixin B followed by Colistin(92.85%). Cotrimoxazole was 100% resistant to these isolates followed by Azithromycin (92.85%.

Conclusion: Klebsiella pneumomiae, Acinetobacter baumanii were the most common bacterial pathogens isolated from



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