Comparative evaluation of geneXpert MTB/RIF assay and Ziehl-Neelsen staining for the diagnosis of tuberculosis
Vol. 11 No. 3 (2022), Original Research Article
Vol. 11 No. 3 (2022)

Comparative evaluation of geneXpert MTB/RIF assay and Ziehl-Neelsen staining for the diagnosis of tuberculosis

Original Research Article
Published 2022-10-01
Deependra Hamal
Manipal College of Medical sciences
Rajani Shrestha
Manipal College of Medical Sciences, Pokhara, Nepal
Sulochana Parajuli
Manipal College of Medical Sciences, Pokhara, Nepal
Niranjan Nayak
Manipal College of Medical Sciences, Pokhara, Nepal
Dharm Raj Bhatt
Manipal College of Medical Sciences, Pokhara, Nepal
Supram Hosuru Subramanya
Manipal College of Medical Sciences, Pokhara, Nepal
Shishir Gokhale
Manipal College of Medical Sciences, Pokhara, Nepal
pdf

Keywords

Microscopy
Mycobacterium tuberculosis
Polymerase chain reaction
Sputum

How to Cite

Hamal, D., Shrestha, R., Parajuli, S. ., Nayak, N. ., Bhatt, D. R. ., Subramanya, S. H., & Gokhale, S. . (2022). Comparative evaluation of geneXpert MTB/RIF assay and Ziehl-Neelsen staining for the diagnosis of tuberculosis. Journal of Kathmandu Medical College, 11(3), 160–164. Retrieved from https://jkmc.com.np/ojs3/index.php/journal/article/view/1160
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Abstract

Background: Ziehl-Neelsen (ZN) staining for the diagnosis of tuberculosis is frequently used. Since ZN smear technique has poor sensitivity, molecular tool like GeneXpert assay have recently been introduced in Nepal. This study compare

pdf

References

Chakaya J, Khan M, Ntoumi F, Aklillu E, Fatima R, Mwaba P and et al. Global tuberculosis report 2020 – Reflections on the global TB burden, treatment and prevention efforts. Int J Infect D. 2021;113 Suppl 1 (Suppl 1):S7-12. [PubMed | Full Text | DOI]

Parsons LM, Somoskövi A, Gutierrez C, Lee E, Paramasivan CN, Abimiku A, Spector S, Roscigno G, Nkengasong J. Laboratory diagnosis of tuberculosis in resource-poor countries: challenges and opportunities. Clin Microbiol Rev. 2011 Apr;24(2):314- 50. [PubMed | Full Text | DOI]

Mechal Y, Benaissa E, Mrimar EN, Benlahlou Y, Bssaibis F, Zegmout A et al. Evaluation of GeneXpert MTB/RIF system performances in the diagnosis of extrapulmonary tuberculosis. BMC Infect Dis. 2019 Dec 19, 1069:1-8. [Full Text | DOI]

Elizabeth R, Stephen L Mouser. Technique of lumbar puncture. In; Harrison’s Principles of Internal Medicine. 19th Ed, New York, Mc Graw Hill education. 443e-2-3p. [Full Text]

RNTCP Central TB Division. Module for Laboratory Technicians, New Delhi, India, Directorate General of Health Services, Ministry of Helath and Family Welfare. 2nd Edition, 2005, Oct :12-24. [Full Text]

WHO XpertMTB/RIF implementation manual: technical and operational “how to” practical consideration. WHO Press, France: GPS Publishing, 2014. [Full Text]

Tostmann A, Kik SV, Kalisvaart NA, Sebek MM, Verver S, Boeree MJ, et al. Tuberculosis transmission by patients with smear-negative pulmonary tuberculosis in a large cohort in the Netherlands. Clin Infect Dis. 2008 Nov 1;47(9):1135-42. [PubMed | Full Text | DOI]

Agarwal M, Bajaj A, Bhatia V, Dutt S. Comparative Study of GeneXpert with ZN Stain and Culture in Samples of Suspected Pulmonary Tuberculosis. J Clin Diagn Res. 2016 May;10(5): 9-12. [PubMed | Full Text | DOI]

Piersimoni C, Scarparo C, Piccoli P, Rigon A, Ruggiero G, Nista D, Bornigia S. Performance assessment of two commercial amplification assays for direct detection of Mycobacterium tuberculosis complex from respiratory and extrapulmonary specimens. J Clin Microbiol. 2002;40: 4138-42. [Full Text | DOI]

Munir MK, Rehman S, Aasim M, Iqbal R, Saeed S. Comparison of Ziehl-Neelsen microscopy with GeneXpert for detection of Mycobacterium tuberculosis. IOSR J Dent and Med Sci 2015;14(11):56- 60. [Full Text | DOI]

Nakata P, Patil S, Patil S, Purohit H, Shelke Y. Comparison of diagnostic efficacy of GeneXpert assay with Ziehl-Neelsen staining and microscopy in diagnosis of pulmonary tuberculosis. IP Int J of Med Microbiol and Trop Dis. 2019;5(4):218-21. [Full Text | DOI]

Chinedum OK, Emwiomwan A, Ifeanyi OE, Babayi A. Comparative Analysis of Ziehl-Neelsen and GeneXpert Techniques for the Diagnosis of Tuberculosis in Human Immunodeficiency Virus Positive Patients in Benin City. Ann of Clin and lab Res. 2017;5(4):1-6. [Full Text | DOI]

Umair M, Siddiqui S, Farooq M. Diagnostic Accuracy of Sputum Microscopy in Comparison with GeneXpert in Pulmonary Tuberculosis. Cureus. 2020 Nov 8;12(11):e11383. [PubMed | Full Text | DOI]

Mvelase NR, Mlisana KP. Xpert MTB/XDR for rapid detection of drug-resistant tuberculosis beyond rifampicin. The Lancet. 2022 Feb 1;2(22):156-7. [PubMed | Full Text | DOI]