Keywords
Pus
Staphylococcus aureus
How to Cite
Abstract
Background: Pus is a thick, white to yellow fluid that consists of dead leucocytes, cellular debris, necrotic tissues, and bacteria characteristic of pyogenic infections that may be exogenous or endogenous. The resistant pathogens isolated from wound infections have become a global challenge and a grave threat to the public health worldwide.
Objectives: To characterise the bacterial isolates from clinical specimens of pyogenic wound infections and to determine the antibiotic susceptibility test.
Methods: A hospital-based, descriptive, cross-sectional study was carried out after ethical clearance in the department of microbiology, Nobel Medical College from 2021 May to 2022 January. Various clinical specimens were obtained by convenience sampling. Identification and antibiotic sensitivity test were done as per the standard microbiological procedures. Data were analysed by SPSS v.20.
Results: Out of 1704 sample collected, 901 (52.8%) showed growth in which 893 (52.4%) showed monomicrobial growth and 8 (0.46%) showed mixed growth. Among the samples, females outnumbered males (F: M-1.4:1) and majority of infection were observed in age group between 21-30 years. Most of the isolates were Gram-negative bacteria (462, 50.8%) which were mostly sensitive to amikacin. The most predominant organism was Staphylococcus aureus (427, 47%) which was 100% sensitive to vancomycin and highly resistant to ampicillin (341, 79.8%).
Conclusion: The dryness in the pipeline of new antibiotic and emergence of multidrug resistant strains have pointed the current need towards active microbial surveillance in all clinical settings and prudent use of antibiotics.
References
Kumar M, Lakra N, Sharma AK, Prasad A, Seema K. Bacterial profile and antibiogram of pus isolates in a tertiary care hospital. Journal of Dental and Medical Sciences. 2020;60:39-45. [Full Text | DOI]
Khanam RA, Islam MR, Sharif A, Parveen R, Sharmin I, Yusuf MA. Bacteriological profiles of pus with antimicrobial sensitivity pattern at a teaching hospital in dhaka city. Bangladesh Journal of Infectious Diseases. 2018;5:10-4. [Full Text | DOI]
Nirmala S, Sengodan R. Aerobic bacterial isolates and their antibiotic susceptibility pattern from pus samples in a tertiary care government hospital in tamilnadu, india. Int J Curr Microbiol App Sci. 2017;6:423-42. [Full Text | DOI]
Giri K, Gurung S, Subedi S, Singh A, Adhikari N. Antibiotic susceptibility pattern of bacterial isolates from soft tissues infection among patients visiting birendra military hospital, chhauni, kathmandu. Tribhuvan University Journal of Microbiology. 2019;6:119-26. [Full Text | DOI]
Upreti N, Rayamajhee B, Sherchan SP, Choudhari MK, Banjara MR. Prevalence of methicillin resistant staphylococcus aureus, multidrug resistant, and extended spectrum ?-lactamase producing gram-negative bacilli causing wound infections at a tertiary care hospital of nepal. Antimicrob Resist Infect Control. 2018;7:121. [PubMed | Full Text | DOI]
Sudhaharan S, Kanne P, Chavali P, Vemu L. Aerobic bacteriological profile and antimicrobial susceptibility pattern of pus isolates from tertiary care hospital in india. J Infect Dev Ctries. 2018;12:842-8. [PubMed | Full Text | DOI]
Chaudhary P, Shakya C, Pokhrel SR, Karki S, Shrestha B, Timalsina B. Prospective study on bacterial isolates with their antibiotic susceptibility pattern from pus (wound) sample in kathmandu model hospital. International Journal of Medicine and Biomedical Sciences. 2015;1:15-22. [Full Text | DOI]
Rijal BP, Satyal D, Parajuli NP. High burden of antimicrobial resistance among Bacteria causing pyogenic wound infections at a tertiary care hospital in kathmandu, nepal. J Pathog. 2017;2017:9458218. [PubMed | Full Text | DOI]
Biradar A, Farooqui F, Prakash R, Khaqri SY, Itagi I. Aerobic bacteriological profile with antibiogram of pus isolates. Indian J Microbiol Res. 2016;3:245-9. [Full Text | DOI]
Pandeya U, Raut M, Bhattarai S, Bhatt PR, Dahal PR. Bacteriological profile and antibiogram of bacterial isolates from pus samples in tertiary care hospital of kathmandu. Tribhuvan University Journal of Microbiology. 2017;4:55-62. [Full Text | DOI]
Mnyambwa NP, Mahende C, Wilfred A, Sandi E, Mgina N, Lubinza C, et al. Antibiotic susceptibility patterns of bacterial isolates from routine clinical specimens from referral hospitals in tanzania: A prospective hospital-based observational study. Infec Drug Resist. 2021;14:869-78. [PubMed | Full Text | DOI]
Muluye D, Wondimeneh Y, Ferede G, Nega T, Adane K, Biadgo B, et al. Bacterial isolates and their antibiotic susceptibility patterns among patients with pus and/or wound discharge at gondar university hospital. BMC Res Notes. 2014;7:619. [PubMed | Full Text | DOI]
Kumari PH, Rani PU, Vijayalakshmi P. Evaluation of microbiological profile and antibiogram of aerobic bacteria isolated from pus samples. Journal of Medical and Allied Sciences. 2018;1:26-35. [Full Text | DOI]
Trojan R, Razdan L, Singh N. Antibiotic susceptibility patterns of bacterial isolates from pus samples in a tertiary care hospital of punjab, india. Int Microbiol. 2016;2016:9302692. [PubMed | Full Text | DOI]
Simonsen GS, Småbrekke L, Monnet DL, Sørensen TL, Møller JK, Kristinsson KG, et al. Prevalence of resistance to ampicillin, gentamicin, and vancomycin in enterococcus faecalis and enterococcus faecium isolates from clinical specimens and use of antimicrobials in five nordic hospitals. J Antimicrob Chemother. 2003; 512:323-31. [PubMed | Full Text | DOI]